Kinase-two-hybrid: towards the conditional interactome
نویسندگان
چکیده
The dynamics of the protein–protein interaction network and how it responds to biological perturbations remain difficult to assay by most traditional techniques. A novel kinase-dependent yeast two‐hybrid framework by Stelzl and colleagues (Grossmann et al, 2015) provides a new prism to study how tyrosine phosphorylation regulates the changes in the interactome under varying conditions.
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stkeciencem D ow nladed fom The fission yeast Schizosaccharomyces pombe has more metazoan-like features than the budding yeast Saccharomyces cerevisiae, yet it has similarly facile genetics. We present a large-scale verified binary protein-protein interactome network, “StressNet,” based on high-throughput yeast two-hybrid screens of interacting proteins classified as part of stress response and...
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عنوان ژورنال:
دوره 11 شماره
صفحات -
تاریخ انتشار 2015